SPECIAL SECTION: MONOCLONAL ANTIBODIES

MAB PRODUCTION: DESCRIPTION OF METHODS

An increasing variety of non-ascites methods now are available for MAB production, both large scale and small. In vitro methods include: hollow fiber bioreactors, modular minifermenters, standard tissue culture flasks, and gas-permeable tissue culture bags. MABs also have been produced in chicken eggs, and there is even a recent report of MABs produced successfully from plants. Some researchers work on human monoclonal antibodies. The readings in this section describe and compare these various methods.

Small-scale monoclonal antibody production in vitro: Methods and resources. L. Jackson, L. Trudel, and N. Lipman. In: Proceedings of the Production of Monoclonal Antibodies Workshop. Aug. 1999. Edited by J. E. McArdle and C J. Lund. Alternatives Research and Development Foundation.

Commercial production of monoclonal antibodies. Simon Saxby. In: Proceedings of the Production of Monoclonal Antibodies Workshop. Aug. 1999. Edited by J. E. McArdle and C J. Lund. Alternatives Research and Development Foundation.

ILAR report on Monoclonal Antibody Production. A Report of the Committee on Methods of Producing Monoclonal Antibodies. Institute for Laboratory Animal Research, National Research Council. 1999.
(PDF file)

Effective monoclonal antibodies produced successfully from plants. Altnews, Dec. 1998.

The Report and Recommendations of ECVAM Workshop 35: The Production of Polyclonal Antibodies in Laboratory Animals. March 1998.

Hollow fiber bioreactors: An alternative to the use of mice for monoclonal antibody production. N.S. Lipman and L.R. Jackson. In: CAAT Technical Report #8: Alternatives in Monoclonal Antibody Production. Sept. 1997.

Comparison of hollow fiber bioreactors and modular minifermentors for the production of MABs in vitro. E. Kagan, E. Viera and H.T. Petrie. In: CAAT Technical Report #8: Alternatives in Monoclonal Antibody Production. Sept. 1997.

Monoclonal antibody production in gas-permeable tissue culture bags using serum-free media. J.R. Heidel and B.V. Stang. In: CAAT Technical Report #8: Alternatives in Monoclonal Antibody Production. Sept. 1997.

Core laboratories: What is their cost-effectiveness and what are their needs? Margaret Penno. In: CAAT Technical Report # 8: Alternatives in Monoclonal Antibody Production. Sept. 1997.

AWIC Information Resources for Adjuvant and Antibody Production: Comparisons and Alternative Technologies (1990-97): Antibody Production Bibliography.

The Report and Recommendations of ECVAM Workshop 21: The Production of Avian (Egg Yolk) Antibodies: IgY. March 1996.

Immunisation of chickens with the aminoterminal propeptide of bovine procollagen type III (specificity of egg yolk antibodies and comparison with immunoassays using rabbit and mouse antibodies). [abstract] M. Gerl, C. Steinert, M. Quint, R. Schade, V. Gunzler. ALTEX, 1996, Supplement.

Development of human monoclonal antibodies. [abstract] Toni Lindl. ALTEX, 1995, vol. 1.

Production of monoclonal antibodies in chicken eggs.
[abstract] A. Hlinak, U. Marx, and V. Jager. ALTEX, 1994, vol. 2.

Monoclonal antibodies: Comparative methods for in vitro production. R. W. Fischer and P. C. Ferber) [abstract] ALTEX, 1992, vol. 1.

Monoclonal antibodies: In vivo and in vitro production on a laboratory scale, with consideration of the legal aspects of animal protection. [abstract] I. Kuhlmann, W. Kurth, and I. Ruhdel. ATLA 17, 73-82, 1989.

Monoclonal antibodies: In vivo and in vitro production on a laboratory scale, with consideration of the legal aspects of animal protection [abstract] I. Kuhlmann, W. Kurth, and I. Ruhdel. ALTEX, 1989, vol. 2.

Mass production of monoclonal antibodies: Technical, scientific and ethical considerations [abstract] Toni Lindl. ALTEX, 1988, vol. 1.

For additional sources, see the In Vitro Methods segment segment of the UCCAA section on Readings and Resources on Monoclonal Antibodies and the Bibliographies section.

See also the Production Bibliography section of the AWIC web site.