Table VII: Maximal Volume (ml) of Freund's Complete Adjuvant Containing Inoculum per Injection Sitea

s.c. i.m. i.p. i.d. f.p.
Guideline Rab.b R.c M.d Rab. R. M. Rab. R. M. Rab. R. M. Rab. R. M.
ANZCCART 0.25 .25 0.2 0.1 0.5 0.1 0.1 0.05 nr nr np 0.05 0.05
ATBW 0.1 0.1 0.1 0.02 0.05 np np npu npu
BVET 0.1 0.1 0.1 0.2 0.2 np npu npu
CCAC 0.25 0.1 0.1 0.5 nr nr np 0.1 0.1 0.05 nr np np npu npu
HO 0.25 0.2 0.1
NIH 0.1 0.1 0.1 0.5 0.05 nr npu npu
TVT 0.1 0.1 0.1 0.5 nr nr 0.2 0.2 0.05 npu npu npu
VPHI 0.1 0.1 0.1 0.5 0.2 0.2 0.05 np np npu npu

ANZCCART = Australian and New Zealand Council for the Care of Animals in Research and Teaching (Australia/New Zealand; 50), ATBW = Arbeitsgemeinschaft der Tierschutzbeauftragten in Baden-Württemberg (Germany; 51), BVET = Bundesamt für Veterinärwesen (Switzerland; 15), CCAC = Canadian Council on Animal Care (Canada; 30), HO = Home Office (UK; 47), NIH = National Institutes of Health (USA; 46), TVT = Tierärzliche Vereinigung für Tierschutz (Germany; 22), VPHI = Veterinary Public Health Inspectorate (The Netherlands; 31).

s.c. = subcutaneous, i.m. = intramuscular, i.p. = intraperitoneal, i.d. = intradermal, f.p. = footpad.

nr = not recommended, n.p. = not permitted, npu = not permitted unless.

aMaximum volumes of inoculum without an adjuvant can be found in several handbooks (23, 38, 52, 53).

bRabbit, cRat, dMouse