ECVAM 35 Table 4

Table IV: Maximum Volume of Injection and Needle Size Used for Injection of Antigen without Adjuvant per Route of Injection for Different Animal Species

	s.c.		i.m.		i.d.		i.p.		i.v.
Species	Vol.^a	Needle^b	Vol.	Needle	Vol.	Needle	Vol.	Needle	Vol.	Needle
Mice	0.5	< 25G	0.05	25-27G	0.05	26G	1	< 23G	0.2	< 25G
Hamsters	1	25G	0.1	< 25G		26G	2-3	< 23G	0.3	< 25G
Guinea-Pigs	1	< 20G	0.1	25G	0.05	26G	10	23G	0.5	< 27G
Rats	1	23-25G	0.1	25G	0.05	26G	5	23G	0.5	25-27G
Rabbits	1.5	20G	0.5	23G	0.05	26G	20	21G	1-5	20-21G
Sheep or goats	5	20G			0.05	26G	10	21G	30
Pigs (> 50kg)	3	19-21G	2	20G	0.1	26G	250	20G	20	21-23G
Chickens	4	23G	0.5	21-23G		26G	10	21G	0.5^c	23G

s.c = subcutaneous, i.m. = intramuscular, i.p. = intraperitoneal, i.d. = intradermal, i.v. = intravenous.

^aMaximum injection volume in ml.

^bNeedle size in G (gauge: 27G = 0.40 mm, 26G = 0.45mm, 25G = 0.50 mm, 24G = 0.55 mm, 23G = 0.60 mm, 22G = 0.70, 21G = 0.80 mm, 20G = 0.90 mm, 19G = 1.00 mm).

^cMI/kg body weight.

Data from references 26 and 27.