ALTEX :: Alternatives to Animal Experiments

1994, Volume 2

Production of Monoclonal Antibodies in Chicken Eggs

Andreas Hlinak¹, Uwe Marx² and Volker Jäger³

¹Institut für Virologie, FB Veterinärmedizin (Standort Mitte) der Freien Universität D-Berlin; ²Institut für Medizinische Immunologie, Universitätsklinikum (Charité) der Humboldt-Universität D-Berlin; ³Gesellschaft für Biotechnologische Forschung mbH, D-Braunschweig

SUMMARY

The possibility to produce monoclonal antibodies in chicken eggs was shown. Knowledge of biochemical and biophysical parameters of eggs were the basic of the experiments. The cell clones produced 0.1 mg/ml of antibodies in the egg fluid. This method can be a alternative to the monoclonal antibody production in mouse ascites or in bioreactors.

Keywords: monoclonal antibody, hybridoma technique, chicken egg, alternative antibody production