ALTEX :: Alternatives to Animal Experiments

1996, Volume 4

An ELISA for the Qunatification of Chicken Immunoglobulin (IgY) in Various Liquid Media

Matthias Fischer and Andreas Hlinak

Institute of Virology, Department of Veterinary Medicine, Free University of D-Berlin

SUMMARY

The development of an assay for the quantification of chicken immunoglobulin (IgY) and data evaluation is being demonstrated. An enzyme immunoassay was established to perform the quality control of IgY purifications from the egg yolk and to control IgY producing cells in vitro. Each step of the test procedure and possible alternatives were described. The assay was characterized by special test parameters (sensitivity, detectability, accuracy, precision, specificity). Moreover, this simple and fast enzyme immunoassay is a special supplement to the alternative antibody production from egg yolk. (article in English)

Keywords: enzyme immunoassay, quantification, chicken immunoglobulin, IgY