Andreas Hlinak¹, Monika Krüger³, Thomas Bartels², Mirko Sasse³, Marina Claros¹, and Rüdiger Schade¹

¹Institute of Virology and ²Institute of Veterinary Pathology, Department of Veterinary Medicine, Free University, D-Berlin; ³Institute of Microbiology and Control of Animal Diseases, Veterinary Faculty, University, D-Leipzig

SUMMARY

The present paper describes the methods for producing egg yolk antibodies against Bordetella bronchiseptica. In comparative experiments, avian and mammalian antibodies were applicated in diagnostic test systems. The results of these investigations show the possibility of substituting mammalian antibodies for IgY in enzyme immunoassay and indirect immunofluorescence. (article in english)

Keywords: egg yolk antibody, IgY, Bordetella bronchiseptica, enzyme immunoassay, immunohistochemistry