ALTEX :: Alternatives to Animal Experiments

1996, Supplement

Production of Egg Yolk Antibodies Against Human Cell-Associated Antigens

Andreas Hlinak¹, Wieland Schrödl², Sabine Witt³, Rüdiger Schade¹, and Monika Krüger⁴

¹Institute of Virology, Department of Veterinary Medicine, Free University, D-Berlin; ²IMTOX GmbH, D-Berlin; ³Department of Medical Immunology, Ernst-Moritz-Arndt-University, D-Greifswald; ⁴Institute of Microbiology and Control of Animal Disease, Veterinary Faculty, Universtity, D-Leipzig

SUMMARY

The aim of this study was the production of specific IgY against cell-associated, highly conserved mammalian proteins. CD 14 is expressed on monocyte surfaces and was identified as endotoxin receptor. P 23 is a cellular protein with unclear biological function. The induction, preparation and characterization of egg yolk antibodies against these antigens are described. (article in english)

Keywords: egg yolk antibody, IgY, cell-associated antigens