ALTEX :: Alternatives to Animal Experiments

1998, Supplement

Cytokine Release after Administration of Endotoxin Containing Vaccines

Martina Ecker and Guenter Mueller

Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinaermedizin, D-Jena

SUMMARY

Endotoxins from gram negative bacteria are known to be potent inducers for the synthesis and the release of cytokines such as tumour necrosis factor (TNF) and interleukin 6 (IL-6). The amount of these proinflammatory mediators in plasma from animals and human patients suffering from an acute infection or sepsis, however, is well correlated with the outcome and the prognosis of such diseases (Hack et al, 1989; Ostermann et al, 1997; Rigato, 1996). In connection with regular testing of vaccine lots, we determine the release and the kinetic of TNF and IL-6 in piglets after immunisation with different vaccines containing endotoxin. The current results suggest that the amounts of both cytokines increased with elevated endotoxin concentration given with the doses. TNF peaked in plasma after one hour, IL-6 peaked between two and four hours per application. We did not find any influence of the gender of the animals. In contrast, the body weight seems to affect the cytokine release in different ways. Determination of cytokine changes in plasma is a sensitive tool for the evaluation of systemic reactions and supports data about the clinical and hematological signs.

Keywords: vaccines, TNF, interleukin 6, swine