ATLA::Alternatives to Laboratory Animals

Volume 24, Number 4

The capacity of murine epithelial cell lines with different degrees of malignant transformation to metabolise benzo[a]pyrene.

ATLA 24, 541-546, July/August 1996

Cristina Viezzer,¹ Roberto Revoltella² and Lucia Celotti¹

¹Dipartimento di Biologia, via Trieste 75, 35100 Padova, Italy; ²Istituto di Mutagenesi e Differenziamento, CNR, via Svezia 2A, 56124 Pisa, Italy

SUMMARY

Cultures of three murine cell lines derived from fetal mouse liver (FL,FLT3 and FLT5) were treated with benzo[a]pyrene to evaluate their ability to activate promutagens. DNA adducts were detected in an three cell lines when they were treated with the major active metabolite of benzo[a]pyrene, benzo[a]pyrene diolepoxide. DNA adducts were also produced in FL and FLT3 cells when they were treated with the promutagen, benzo[a]pyrene, while treatment of FLT5 cells resulted in very few DNA adducts. In treated FL and FLT3 cents, the most evident adduct spot detected showed a chromatographic position similar to that of the main adduct formed in cells treated with (±)-r-7,t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene.

Keywords: metabolic activation, DNA abduct, benzo[a]pyrene