ATLA::Alternatives to Laboratory Animals

Volume 25, Number 3

Cytotoxic and genotoxic effects of sodium fluoride on mammalian cells at neutral or acid pH: a summary of published work.

ATLA 25, 341-342, May/June 1997

Darina Slameñová, Alena Gábelová1 Katarina Ruppová2 and Ladislave WsóLová2

1Cancer Research Institute of Slovak Academy of Sciences, Spitálska 21, 81232 Bratislava, Slovak Republic; 2Institute of Preventative and Clinical Medicine, Limbová 14, 83101 Bratislava, Slovak Republic

SUMMARY

This paper forms a short review of some of our previously published work. We assessed the cytotoxic and genotoxic effects of sodium fluoride (NaF) on the Chinese hamster V79 cell line and the human EUE cell line at neutral and acid pH (as found in the stomach). The effects of NaF on cell proliferation, DNA synthesis, protein synthesis and mutagenesis were assessed. The results found showed no mutagenic effects after exposure to NaF, despite some cytogenic effects, suggesting that NaF probably does not represent any senous mutagenic or carcinogenic threat to humans.

Keywords: hamster V79 cell line, human EUE cell line, sodium fluoride, cytotoxicity, genotoxicity