Local Lymph Node Assay

Table of Contents

  1. Test Submission
    1. Cover Letter
    2. Submission
  2. Appendices
    1. Local Lymph Node Assay Bibliography
    2. List of Chemicals
      1. Chemicals Tested in Local Lymph Node Assay
      2. Discordant Results Between Local Lymph Node Assay and Guinea Pig or Human Test Methods
      3. Disintegrations Per Minute Data and Stimulation Indices for Discordant Results
    3. Key Local Lymph Node Assay Papers
    4. Sample Local Lymph Node Assay Protocol
    5. ICCVAM Local Lymph Node Assay Test Submission Guidelines

APPENDIX B: Table 1

Chemicals Tested in Local Lymph Node Assay

Chemical CAS Number LLNA GPMT/BT# HMT MEST
Abietic acid 514-10-3 + +
2-(N-acetoxy-acetamino)fluorene   +
3-Acetylphenylbenzoate   + +
4-Allylanisole 140-67-0 + +
2-Aminophenol 95-55-6 + +*
3-Aminophenol 591-275 + +*
Ammonium tetrachloroplatinate 13820-41-2
+
+
Ammonium thioglycolate 5421-46-5 + -
Aniline 62-53-3 + + +
Benzene-1,3,4-tricarboxylic anhydride   + +
1,2-Benzisothiazolin-3-one   + +
Benzo[a]pyrene 50-32-8 +
Benzocaine 94-09-7 +/-** +/-** +
Benzoquinone 106-51-4 + +
Benzoyl chloride 98-88-4 + +
Benzoyl peroxide 94-36-0 + +   +
Benzoyl bromide 100-39-0 +
Beryllium sulphate 7787-56-6 + + +
1-Bromododecane 143-15-7 + +*
12-Bromododecanoic acid 73367-80-3 +
12-Bromo-1-dodecanol 3344-77-2 +
1-Bromohexadecane 112-82-3 + +
1-Bromohexane 111-25-1 + +*
3-Bromomethyl-3- dimethyldihydrofuranone   + +
1-Bromopentadecane 629-72-1 +
7-Bromotetradecane   +
2-Bromotetradecanoic acid 10520-81-7 +
2,3-Butanedione 431-03-8 +
Butylglycidyl ether 2426-08-6 + + +
C12,13-β branched primary alcohol sulphate   +
C16-1,3-alkene sultone   + +*
Camphorquinone 465-29-2 +
Chloroamine T 10599-90-3 + +
4-Chloroaniline 106-47-8 + +
2-Chloromethylfluorene   +
(Chloro)methylisothiazolinone 55965-84-9 + +
1-Chloromethylpyrene 1086-00-6 +
1-Chlorononane 2473-01-0 +
1-Chlorooctadecane 3386-33-2 +
1-Chlorotetradecane 2425-54-9 +
Chloropromazine 69-09-0 + +* +
Cinnamic aldehyde 104-55-2 + + + +
Citral 5392-40-5 + + +
Clotrimazole 22593-75-1 +
Cobalt Chloride 7646-79-9 + + +
Cocoamidopropyl betaine 59141-98-9 + +
Copper chloride 7758-89-6 + -
Dibromodicyanobutane   + +
Diethyl sulphate 64-67-5 +
Diesthylenetriamine 111-40-0 + + +
7,12-Dihydrocoumarin 119-84-6 +
Dihydroeugenol 2785-87-7 + +
3-Dimethylaminopropylamine 109-55-7 + +
7,12-Dimethylbenz[a]anthracene 57-97-6 +
5,5-Dimethyl-3-methylenedihydro- 2(3H)-furanone   + -*
5,5-Dimethyl-3-(thiocyanatomethyl) dihydro-2(3H)-furanone   + +*
Dimethyl sulphate 77-78-1 +
2,4-Dinitrochlorobenzene 97-00-7 + +   +
2,4-Dinitrothiocyanobenzene 1494-56-5 + +
Disodium 1,2-diheptanoyloxy-3,5- benzenedisulphonate   + +*
Dodecylmethanesulphonate 51323-71-8 + +*
Dodecylthiosulphonate   + +
Ellipticine 519-23-3 +
Ethylene diamine 107-15-3 + +   +
Ethylene glycol dimethacrylate 97-90-5 + -
1-Ethyl-3-nitro-1-nitrosoguanidine   +
Eugenol 97-53-0 + +   +
Fluorescein isothiocyanate 25168-13-2 +
Formaldehyde 50-0-0 + + + +
Glyoxal 107-22-2 + + +
Gold chloride 16903-35-8 +   +
Hexadecanoyl chloride 112-67-4 +
Hexyl cinnamic aldehyde 101-86-0 + +
Hydroquinone 123-31-9 + +
Hydroxycitronellel 107-75-5 + + +
2-Hydroxyethyl acrylate 818-61-1 + +
Imidazolidinyl urea 39236-46-9 + +
1-Iodohexadecane 544-77-4 +
1-Iodononane 4282-42-2 +
1-Iodotetradecane 192-94-1 +
Isoeugenol 97-54-1 + +
Isopropylisoeugenol 29653-00-7 + +
Isononanoyloxybenzene sulphonate   + +
Isophorone diisocyanate 4098-71-9 + +
2-Mercaptobenzothiazole 149-30-4 + + +
Mercuric chloride 7487-94-7 + + +
2-Methoxy-4-methyl phenol 5635-98-3 + +
3-Methoxyphenylbenzoate 5554-24-5 +
4-Methylaminophenol sulphate 55-55-0 + +
3-Methylcatechol 488-17-5 +
4-Methylcatechol 452-86-8 + +
3-Methylcholanthrene 56-49-5 +
Methyl dodecane sulphonate   + +
3-Methyleugenol   +
5-Methyleugenol   +
6-Methyleugenol   +
Methyl hexadecane sulphonate   + +*
3-Methyl isoeugenol   + +*
Methyl methane sulphonate 66-27-3 +
1-Methyl-3-nitro-1- nitrosguanidine 70-25-7 +
Methyl(2-sulphomethyl) octadecanoate   +
2-Methyl-4,5-trimethylene-4- isothiazolin-3-one   + +
Musk ambrette 83-66-9 + -
N-Ethyl-N-nitrosourea   +
N-Methyl-N-nitrosourea 684-93-5 +
a-Naphthoflavone 604-59-1 +
β-Naphthoflavone 6051-87-2 +
Neomycin sulphate 1405-10-3 +/- +
4-Nitrobenzyl bromide 100-11-8 + +*
4-Nitrobenzyl chloride 100-14-1 + +*
4-Nitroso-N,N-dimethylaniline 138-89-6 + +
Nonanoyl chloride 764-85-2 +
Octadecanoyl chloride 112-76-5 +
Octyl gallate 1034-01-1 +
Oxazolone 15646-46-5 + +   +
Penicillin G 61-33-6 + + +
Pentachlorophenol 87-86-5 +   +
Phenyl benzoate 93-99-2 + +
3-Phenylenediamine 108-45-2 + +*
4-Phenylenediamine 106-50-3 + + + +
Phthalic anhydride 85-44-9 + +   -
Picryl chloride 88-88-0 + +   -
Polyhexamethylene biguanide   + + + -
Potassium dichromate 7778-50-9 + + + -
β-Propiolactone 55-57-8 +
Propylgallate 121-79-9 + +
1-Propyl-3-nitro-1- nitrosoguanidine   +
Pyridine 110-86-1 +   +/-
Quinol 123-31-9 + +*
Sodium Benzoyloxybenzene sulphonate   + +
Sodium 4-(2-ethylhexyloxycarboxy) benzene sulphonate   + +*
Sodium 4-sulphophenyl acetate   + +*
Sodium benzoyloxy-2-methoxy-5- benzene sulphonate   + +*
Sodium lauryl sulphate 151-21-3 + - - -
Sodium nornornanacetoxy-4-benzene sulphonate   + +*
Streptomycin 57-92-1 + +
Tetrachlorosalicylanilide 7426-07-5 + + +
Tetradecul iodide 19218-94-1 +
Tetramethyl thiuram disulphide 137-26-8 + +* +
1-Thioglycerol 96-27-5 + + +
Toluene diamine bismaleimide   + +
2,4,5-Trichlorophenol 95-95-4 +
2,4,6-Trichloro-1,3,5- triazine 87-90-1 +
a-Trimethylammonium-4-tolyloxy- 4-benzene sulphonate   + +*
3,5,5-Trimethylhexanoyl chloride 36727-29-4 + +
Vinyl pyridine 1337-81-1 +
Xylene 1330-20-7 +   -
Zinc sulfate 7733-02-0 +
2-Acetamidofluorene 53-96-3 -
4-Acetylphenylbenzoate 1523-18-8 -
4-Aminobenzoic acid 150-13-0 - - - +
Benzalkonium chloride 8001-54-5 - -   +
3-(Benzenesulphonyloxymethyl)- 5,5-dimethyldihydro-2(3H)- furanone   -
Benzoic acid 65-85-0 - -
+
Benzoyloxy-3,5 benzene dicarboxylic acid   - +*
1-Bromobutane 109-65-9 -
Chlorobenzene 108-90-7 - -
3-(Chlorobenzenesulphonyloxymethyl) -5,5-dimethyl dihydro-2(3H)-furanone   -
2-Chloroethanol 107-07-3
Dextran 9004-54-0 - -
2,4-Dichloronitrobenzene 611-06-3 - -
Di-2-furanylethanedione 492-94-4 -
5,5-Dimethyl-3-(mesyloxymethyl) dihydro-2(3H)-furanone   - +*
5,5-Dimethyl-3- (methoxybenzenesulphonyloxymethyl) dihydro-2(3H)-furanone   - +*
5,5-Dimethyl-3- (nitrobenzenesulphonyloxymethyl) dihydro-2(3H)-furanone   - +*
Dimethylisophthalate 1459-93 - -
5,5-Dimethyl-3- (tosyloxymethyl)dihydro- 2(3H)-furanone   - -*
Disodium benzoyloxy-3,5- benzenedicarboxylate   - -
Ditallowdihydroxypropenetrimethyl ammonium   - -
Ethylmethanesulphonate 62-50-0 -
Geraniol 106-24-1 - - -
Glycerol 56-81-5 - -   -
Hexane 110-54-3 -   -
Hydrocortisone 50-23-7 -   -
4-Hydroxybenzoic acid 99-96-7 - -
2-Hydroxypropylmethacrylate 923-26-2 - -
Isopropanol 67-63-0 - -
Kanamycon 25389-94-0 - -* +
Lactic Acid 50-21-5 - -
Lanolin 8006-54-0 - -
Lead acetate 15347-57-6 -
6-Methylcoumarin 92-48-8 - - -
Methyl salicylate 119-36-8 - - -
N'-(4-Methylcyclohexyl)-N- (2-chlroethyl)-N-nitrosourea   -
Nickel chloride 7718-54-9 - +
Nickel sulphate 10101-98-1 - + + +
2-Nitrofluorene 607-57-8 -
Octadecylmethane sulphonate 31081-59-1 - +*
Phenol 108-95-2 -   - -
Phthalic acid diethyl ester   -
Propylparaben 94-13-3 - - +/-
Propylene glycol 557-55-6 - -   -
Resorcinol 108-46-3 - - -
Salicylic Acid 69-72-7 - - - -
Streptozotocin 18883-66-4 -
Sulphanilamide 63-74-1 - - +
Sulphanilic acid 121-57-3 - +   +
Tartaric acid 87-69-4 - -*
Tixocortol pivalate 55560-96-8 -
Toluene sulphonamide formaldehyde resin   - -
Trimethylammonium-3- tolyl-E-caprolactimide chloride   -
Tween 80 9005-65-6 - - -

#Positive results based on EC classification threshold
*Result obtained in a non-standard guinea pig test
**Ref Benzocaine paper