Local Lymph Node Assay

Table of Contents

  1. Test Submission
    1. Cover Letter
    2. Submission
  2. Appendices
    1. Local Lymph Node Assay Bibliography
    2. List of Chemicals
      1. Chemicals Tested in Local Lymph Node Assay
      2. Discordant Results Between Local Lymph Node Assay and Guinea Pig or Human Test Methods
      3. Disintegrations Per Minute Data and Stimulation Indices for Discordant Results
    3. Key Local Lymph Node Assay Papers
    4. Sample Local Lymph Node Assay Protocol
    5. ICCVAM Local Lymph Node Assay Test Submission Guidelines

APPENDIX B: Table 3

Disintegrations Per Minute (DPM) Data and Stimulation Indices (SI) for Discordant Results

Chemical Concentration1 (%) DPM SI
Hexyl cinnamic aldehyde Vehicle (AOO) 495 1.0
Example of positive LLNA response 2.5 691 1.4
  5.0 1056 2.1
  10.0 1615 3.3
  25.0 4107 8.3
  50.0 6857 14.0
para-Aminobenzoic acid Vehicle (AOO) 453 1.0
Example of negative LLNA response 0.5 399 0.9
  1.0 457 1.0
  2.5 626 1.4
  5.0 519 1.1
  10.0 452 1.0
Ammonium thioglycolate Vehicle (DMSO) 807 1.0
  10.0 2389 3.0
  25.0 2490 3.1
  50.0 3250 4.0
Benzocaine Vehicle (DMF) 325 1.0
  2.5 562 1.7
  5.0 574 1.8
  10.0 698 2.1
  25.0 794 2.4
Copper chloride Vehicle (DMSO) 605 1.0
  1.0 4920 8.1
  2.5 8341 13.8
  5.0 8225 13.6
5,5-Dimethyl-3-methylenedihydro -2(2H)-furanone Vehicle (AOO) 672 1.0
  1.77 2022 3.0
  3.53 5002 7.4
  7.06 6213 9.2
Ethylene glycol dimethacrylate Vehicle (Acetone) 365 1.0
  10.0 675 1.8
  25.0 1312 3.6
  50.0 4046 11.1
Neomycin sulphate Vehicle (DMSO) 355 1.0
  25.0 379 1.1
Pyridine Vehicle (AOO) 250 1.0
  25.0 274 1.1
  50.0 578 2.3
  100.0 978 3.9
Sodium lauryl sulphate Vehicle (DMF) 369 1.0
  1.0 747 2.0
  2.5 954 2.6
  5.0 1301 3.5
  10.0 1814 4.9
  20.0 1628 4.4
Xylene Vehicle (AOO) 382 1.0
  25.0 487 1.3
  50.0 1138 3.0
  100.0 1182 3.1
Benzoyloxy-3,5-benzne dicarboxylic acid Veh. (Ace/Sal) 382 1.0
  2.5 346 0.9
  5.0 315 0.8
  10.0 419 1.1
5,5-Dimethyl-3-(methylbenzenesulpho- nyloxymethyl)dihydro-2(3H)-furanone Vehicle (AOO) 526 1.0
  3.42 494 0.9
  6.83 791 1.5
  13.66 702 1.3
5,5-Dimethyl-3-(methoxybenzenesulpho- nyloxymethyl)dihydro-2(3H)-furanone Vehicle (AOO) 672 1.0
  4.84 802 1.2
  9.67 612 0.9
  19.34 690 1.0
5,5-Dimethyl-3-(nitrobenzenesulphonyl- oxymethyl)dihydro-2(3H)-furanone Vehicle (AOO) 657 1.0
  5.07 493 0.8
  10.13 490 0.7
  20.26 585 0.9
Hydrocortisone Vehicle (AOO) 250 1.0
  2.5 74 0.3
  5.0 29 .01
  10.0 16 0.06
Kanamycin Vehicle (AOO) 382 1.0
  5.0 842 2.2
  10.0 301 0.8
  25.0 391 1.0
Nickel chloride Vehicle (DMSO) 898 1.0
  1.0 1363 1.5
  2.5 1940 2.2
  5.0 2133 2.4
Nickel sulphate Vehicle (DMSO) 898 1.0
  0.5 986 1.1
  1.0 1315 1.5
  2.5 1376 1.5
Octadecylmethane sulphonate Vehicle (AOO) 510 1.0
  2.5 594 1.2
  5.0 374 0.7
  10.0 444 0.9
Propylparaben Vehicle (AOO) 433 1.0
  10.0 595 1.4
  25.0 445 1.0
  50.0 575 1.3
Sulphanilamide Vehicle (DMF) 416 1.0
  10.0 429 1.0
  25.0 415 1.0
  50.0 393 0.9
Sulphanilic acid Vehicle (DMSO) 436 1.0
  2.5 667 1.5
  5.0 827 1.9
  10.0 967 2.2

1Abbreviations Used: