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Local Lymph Node Assay

Table of Contents

  1. Test Submission
    1. Cover Letter
    2. Submission
  2. Appendices
    1. Local Lymph Node Assay Bibliography
    2. List of Chemicals
      1. Chemicals Tested in Local Lymph Node Assay
      2. Discordant Results Between Local Lymph Node Assay and Guinea Pig or Human Test Methods
      3. Disintegrations Per Minute Data and Stimulation Indices for Discordant Results
    3. Key Local Lymph Node Assay Papers
    4. Sample Local Lymph Node Assay Protocol
    5. ICCVAM Local Lymph Node Assay Test Submission Guidelines

APPENDIX B: Table 1

Chemicals Tested in Local Lymph Node Assay

ChemicalCAS NumberLLNAGPMT/BT#HMTMEST
Abietic acid514-10-3++
2-(N-acetoxy-acetamino)fluorene +
3-Acetylphenylbenzoate ++
4-Allylanisole140-67-0++
2-Aminophenol95-55-6++*
3-Aminophenol591-275++*
Ammonium tetrachloroplatinate13820-41-2
+
+
Ammonium thioglycolate5421-46-5+-
Aniline62-53-3+++
Benzene-1,3,4-tricarboxylic anhydride ++
1,2-Benzisothiazolin-3-one ++
Benzo[a]pyrene50-32-8+
Benzocaine94-09-7+/-**+/-**+
Benzoquinone106-51-4++
Benzoyl chloride98-88-4++
Benzoyl peroxide94-36-0++ +
Benzoyl bromide100-39-0+
Beryllium sulphate7787-56-6+++
1-Bromododecane143-15-7++*
12-Bromododecanoic acid73367-80-3+
12-Bromo-1-dodecanol3344-77-2+
1-Bromohexadecane112-82-3++
1-Bromohexane111-25-1++*
3-Bromomethyl-3- dimethyldihydrofuranone ++
1-Bromopentadecane629-72-1+
7-Bromotetradecane +
2-Bromotetradecanoic acid10520-81-7+
2,3-Butanedione431-03-8+
Butylglycidyl ether2426-08-6+++
C12,13-? branched primary alcohol sulphate +
C16-1,3-alkene sultone ++*
Camphorquinone465-29-2+
Chloroamine T10599-90-3++
4-Chloroaniline106-47-8++
2-Chloromethylfluorene +
(Chloro)methylisothiazolinone55965-84-9++
1-Chloromethylpyrene1086-00-6+
1-Chlorononane2473-01-0+
1-Chlorooctadecane3386-33-2+
1-Chlorotetradecane2425-54-9+
Chloropromazine69-09-0++*+
Cinnamic aldehyde104-55-2++++
Citral5392-40-5+++
Clotrimazole22593-75-1+
Cobalt Chloride7646-79-9+++
Cocoamidopropyl betaine59141-98-9++
Copper chloride7758-89-6+-
Dibromodicyanobutane ++
Diethyl sulphate64-67-5+
Diesthylenetriamine111-40-0+++
7,12-Dihydrocoumarin119-84-6+
Dihydroeugenol2785-87-7++
3-Dimethylaminopropylamine109-55-7++
7,12-Dimethylbenz[a]anthracene57-97-6+
5,5-Dimethyl-3-methylenedihydro- 2(3H)-furanone +-*
5,5-Dimethyl-3-(thiocyanatomethyl) dihydro-2(3H)-furanone ++*
Dimethyl sulphate77-78-1+
2,4-Dinitrochlorobenzene97-00-7++ +
2,4-Dinitrothiocyanobenzene1494-56-5++
Disodium 1,2-diheptanoyloxy-3,5- benzenedisulphonate ++*
Dodecylmethanesulphonate51323-71-8++*
Dodecylthiosulphonate ++
Ellipticine519-23-3+
Ethylene diamine107-15-3++ +
Ethylene glycol dimethacrylate97-90-5+-
1-Ethyl-3-nitro-1-nitrosoguanidine +
Eugenol97-53-0++ +
Fluorescein isothiocyanate25168-13-2+
Formaldehyde50-0-0++++
Glyoxal107-22-2+++
Gold chloride16903-35-8+ +
Hexadecanoyl chloride112-67-4+
Hexyl cinnamic aldehyde101-86-0++
Hydroquinone123-31-9++
Hydroxycitronellel107-75-5+++
2-Hydroxyethyl acrylate818-61-1++
Imidazolidinyl urea39236-46-9++
1-Iodohexadecane544-77-4+
1-Iodononane4282-42-2+
1-Iodotetradecane192-94-1+
Isoeugenol97-54-1++
Isopropylisoeugenol29653-00-7++
Isononanoyloxybenzene sulphonate ++
Isophorone diisocyanate4098-71-9++
2-Mercaptobenzothiazole149-30-4+++
Mercuric chloride7487-94-7+++
2-Methoxy-4-methyl phenol5635-98-3++
3-Methoxyphenylbenzoate5554-24-5+
4-Methylaminophenol sulphate55-55-0++
3-Methylcatechol488-17-5+
4-Methylcatechol452-86-8++
3-Methylcholanthrene56-49-5+
Methyl dodecane sulphonate ++
3-Methyleugenol +
5-Methyleugenol +
6-Methyleugenol +
Methyl hexadecane sulphonate ++*
3-Methyl isoeugenol ++*
Methyl methane sulphonate66-27-3+
1-Methyl-3-nitro-1- nitrosguanidine70-25-7+
Methyl(2-sulphomethyl) octadecanoate +
2-Methyl-4,5-trimethylene-4- isothiazolin-3-one ++
Musk ambrette83-66-9+-
N-Ethyl-N-nitrosourea +
N-Methyl-N-nitrosourea684-93-5+
a-Naphthoflavone604-59-1+
?-Naphthoflavone6051-87-2+
Neomycin sulphate1405-10-3+/-+
4-Nitrobenzyl bromide100-11-8++*
4-Nitrobenzyl chloride100-14-1++*
4-Nitroso-N,N-dimethylaniline138-89-6++
Nonanoyl chloride764-85-2+
Octadecanoyl chloride112-76-5+
Octyl gallate1034-01-1+
Oxazolone15646-46-5++ +
Penicillin G61-33-6+++
Pentachlorophenol87-86-5+ +
Phenyl benzoate93-99-2++
3-Phenylenediamine108-45-2++*
4-Phenylenediamine106-50-3++++
Phthalic anhydride85-44-9++ -
Picryl chloride88-88-0++ -
Polyhexamethylene biguanide +++-
Potassium dichromate7778-50-9+++-
?-Propiolactone55-57-8+
Propylgallate121-79-9++
1-Propyl-3-nitro-1- nitrosoguanidine +
Pyridine110-86-1+ +/-
Quinol123-31-9++*
Sodium Benzoyloxybenzene sulphonate ++
Sodium 4-(2-ethylhexyloxycarboxy) benzene sulphonate ++*
Sodium 4-sulphophenyl acetate ++*
Sodium benzoyloxy-2-methoxy-5- benzene sulphonate ++*
Sodium lauryl sulphate151-21-3+---
Sodium nornornanacetoxy-4-benzene sulphonate ++*
Streptomycin57-92-1++
Tetrachlorosalicylanilide7426-07-5+++
Tetradecul iodide19218-94-1+
Tetramethyl thiuram disulphide137-26-8++*+
1-Thioglycerol96-27-5+++
Toluene diamine bismaleimide ++
2,4,5-Trichlorophenol95-95-4+
2,4,6-Trichloro-1,3,5- triazine87-90-1+
a-Trimethylammonium-4-tolyloxy- 4-benzene sulphonate ++*
3,5,5-Trimethylhexanoyl chloride36727-29-4++
Vinyl pyridine1337-81-1+
Xylene1330-20-7+ -
Zinc sulfate7733-02-0+
2-Acetamidofluorene53-96-3-
4-Acetylphenylbenzoate1523-18-8-
4-Aminobenzoic acid150-13-0---+
Benzalkonium chloride8001-54-5-- +
3-(Benzenesulphonyloxymethyl)- 5,5-dimethyldihydro-2(3H)- furanone -
Benzoic acid65-85-0--+
Benzoyloxy-3,5 benzene dicarboxylic acid -+*
1-Bromobutane109-65-9-
Chlorobenzene108-90-7--
3-(Chlorobenzenesulphonyloxymethyl) -5,5-dimethyl dihydro-2(3H)-furanone -
2-Chloroethanol107-07-3
Dextran9004-54-0--
2,4-Dichloronitrobenzene611-06-3--
Di-2-furanylethanedione492-94-4-
5,5-Dimethyl-3-(mesyloxymethyl) dihydro-2(3H)-furanone -+*
5,5-Dimethyl-3- (methoxybenzenesulphonyloxymethyl) dihydro-2(3H)-furanone -+*
5,5-Dimethyl-3- (nitrobenzenesulphonyloxymethyl) dihydro-2(3H)-furanone -+*
Dimethylisophthalate1459-93--
5,5-Dimethyl-3- (tosyloxymethyl)dihydro- 2(3H)-furanone --*
Disodium benzoyloxy-3,5- benzenedicarboxylate --
Ditallowdihydroxypropenetrimethyl ammonium --
Ethylmethanesulphonate62-50-0-
Geraniol106-24-1---
Glycerol56-81-5-- -
Hexane110-54-3- -
Hydrocortisone50-23-7- -
4-Hydroxybenzoic acid99-96-7--
2-Hydroxypropylmethacrylate923-26-2--
Isopropanol67-63-0--
Kanamycon25389-94-0--*+
Lactic Acid50-21-5--
Lanolin8006-54-0--
Lead acetate15347-57-6-
6-Methylcoumarin92-48-8---
Methyl salicylate119-36-8---
N'-(4-Methylcyclohexyl)-N- (2-chlroethyl)-N-nitrosourea -
Nickel chloride7718-54-9-+
Nickel sulphate10101-98-1-+++
2-Nitrofluorene607-57-8-
Octadecylmethane sulphonate31081-59-1-+*
Phenol108-95-2- --
Phthalic acid diethyl ester -
Propylparaben94-13-3--+/-
Propylene glycol557-55-6-- -
Resorcinol108-46-3---
Salicylic Acid69-72-7----
Streptozotocin18883-66-4-
Sulphanilamide63-74-1--+
Sulphanilic acid121-57-3-+ +
Tartaric acid87-69-4--*
Tixocortol pivalate55560-96-8-
Toluene sulphonamide formaldehyde resin --
Trimethylammonium-3- tolyl-E-caprolactimide chloride -
Tween 809005-65-6---

#Positive results based on EC classification threshold
*Result obtained in a non-standard guinea pig test
**Ref Benzocaine paper

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