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Local Lymph Node Assay

Table of Contents

  1. Test Submission
    1. Cover Letter
    2. Submission
  2. Appendices
    1. Local Lymph Node Assay Bibliography
    2. List of Chemicals
      1. Chemicals Tested in Local Lymph Node Assay
      2. Discordant Results Between Local Lymph Node Assay and Guinea Pig or Human Test Methods
      3. Disintegrations Per Minute Data and Stimulation Indices for Discordant Results
    3. Key Local Lymph Node Assay Papers
    4. Sample Local Lymph Node Assay Protocol
    5. ICCVAM Local Lymph Node Assay Test Submission Guidelines

APPENDIX B: Table 3

Disintegrations Per Minute (DPM) Data and Stimulation Indices (SI) for Discordant Results

ChemicalConcentration1 (%)DPMSI
Hexyl cinnamic aldehydeVehicle (AOO)4951.0
Example of positive LLNA response2.56911.4
 5.010562.1
 10.016153.3
 25.041078.3
 50.0685714.0
para-Aminobenzoic acidVehicle (AOO)4531.0
Example of negative LLNA response0.53990.9
 1.04571.0
 2.56261.4
 5.05191.1
 10.04521.0
Ammonium thioglycolateVehicle (DMSO)8071.0
 10.023893.0
 25.024903.1
 50.032504.0
BenzocaineVehicle (DMF)3251.0
 2.55621.7
 5.05741.8
 10.06982.1
 25.07942.4
Copper chlorideVehicle (DMSO)6051.0
 1.049208.1
 2.5834113.8
 5.0822513.6
5,5-Dimethyl-3-methylenedihydro -2(2H)-furanoneVehicle (AOO)6721.0
 1.7720223.0
 3.5350027.4
 7.0662139.2
Ethylene glycol dimethacrylateVehicle (Acetone)3651.0
 10.06751.8
 25.013123.6
 50.0404611.1
Neomycin sulphateVehicle (DMSO)3551.0
 25.03791.1
PyridineVehicle (AOO)2501.0
 25.02741.1
 50.05782.3
 100.09783.9
Sodium lauryl sulphateVehicle (DMF)3691.0
 1.07472.0
 2.59542.6
 5.013013.5
 10.018144.9
 20.016284.4
XyleneVehicle (AOO)3821.0
 25.04871.3
 50.011383.0
 100.011823.1
Benzoyloxy-3,5-benzne dicarboxylic acidVeh. (Ace/Sal)3821.0
 2.53460.9
 5.03150.8
 10.04191.1
5,5-Dimethyl-3-(methylbenzenesulpho- nyloxymethyl)dihydro-2(3H)-furanoneVehicle (AOO)5261.0
 3.424940.9
 6.837911.5
 13.667021.3
5,5-Dimethyl-3-(methoxybenzenesulpho- nyloxymethyl)dihydro-2(3H)-furanoneVehicle (AOO)6721.0
 4.848021.2
 9.676120.9
 19.346901.0
5,5-Dimethyl-3-(nitrobenzenesulphonyl- oxymethyl)dihydro-2(3H)-furanoneVehicle (AOO)6571.0
 5.074930.8
 10.134900.7
 20.265850.9
HydrocortisoneVehicle (AOO)2501.0
 2.5740.3
 5.029.01
 10.0160.06
KanamycinVehicle (AOO)3821.0
 5.08422.2
 10.03010.8
 25.03911.0
Nickel chlorideVehicle (DMSO)8981.0
 1.013631.5
 2.519402.2
 5.021332.4
Nickel sulphateVehicle (DMSO)8981.0
 0.59861.1
 1.013151.5
 2.513761.5
Octadecylmethane sulphonateVehicle (AOO)5101.0
 2.55941.2
 5.03740.7
 10.04440.9
PropylparabenVehicle (AOO)4331.0
 10.05951.4
 25.04451.0
 50.05751.3
SulphanilamideVehicle (DMF)4161.0
 10.04291.0
 25.04151.0
 50.03930.9
Sulphanilic acidVehicle (DMSO)4361.0
 2.56671.5
 5.08271.9
 10.09672.2

1Abbreviations Used:

  • DMSO = dimethylsulphoxide
  • DMF = dimethylformamide
  • AOO = acetone:olive oil (4:1)
  • Ace/Sal = acetone:saline (1:1)

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