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Adjuvants in Laboratory Animals

Evaluation of immunostimulating properties and side effects of Freund's complete adjuvant and alternative adjuvants in immunization procedures

Printed by: Ponsen & Looijen BV, Wageningen
Authored by: Dr. P.P.A. Marlies Leenaars

The studies presented in this thesis were perfommed at the Division Immunological and Infectious Diseases (I&I), TNO Prevention and Health (TNO-PG), Leiden, the Netherlands, and the Central Animal Laboratory, National Institute of Public Health and the Environment (RIVM), Bilthoven, the Netherlands. The research was financially supported by the RIVM-lnstitute's Centre for Alternatives to Animal Testing, Bilthoven, the Division I&I, TNO-PG, Leiden and the Department of Immunology, Erasmus University, Rotterdam.

The printing of this thesis was financially supported by:
RlVM-lnstitute's Centre for Altematives to Animal Testing, Bilthoven
TNO-PG, Leiden
Erasmus University, Rotterdam


List of abbreviations
Chapter 1Introduction
Chapter 2Antigens and antigen presentation
Immunology Methods Manual, 1997, pp. 989-1013
Chapter 3Evaluation of several adjuvantsas alternatives
to the use of Freund's adjuvant in rabbits
Vet. Immunol. Immunopathol., 1994, 40: 225-241
Chapter 4Comparison of several types of adjuvants for immune
potentiating properties and side effects in mice
Vet Immunol. Immunopathol., 1995, 48: 123-138
Chapter 5Assessment of side effects induced by injection of different
adjuvanVantigen combinations in rabbits and mice
Chapter 6Immune responses and side effects of five different oil
based adjuvants in mice
Vet Immunol. Immunopathol., in press
Chapter 7Model to test efficacy of immunization procedures by
early cytokine production in mice
Animal Altematives Welfare and Ethics, 1997, in press
Chapter 8Increased adjuvant efficacy in stimulation of antibody
responses after macrophage elimination in viva
Immunology, 1997, 90: 337-343
Chapter 9A rapid and simple method to localize oil based
adjuvants in viva
J. Immunol Methods, in press
Chapter 10General discussion

Curriculum vitae
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